Lactobacillus Acidophilus

Lactobacillus acidophilus reference formula with an example 10 mg of probiotic powder per serving, developed as a tablet or a capsule, with the final amount set by the brand.

Lactobacillus Acidophilus is a reference formula for brands building a single-strain probiotic product. The reference labels declare the probiotic in two ways: as the weight of probiotic powder (mg) and as live cells (CFU). The example serving carries 10 mg of Lactobacillus acidophilus powder in one tablet, and the brand sets the strain, the CFU target and the basis of the label for its market.

A capsule is the format with the most reference labels, with tablets close behind, and the example amount comes from a tablet label. The development work centers on the strain and its documented evidence, the CFU basis (at manufacture or through shelf life), and the moisture and temperature protection that live cultures need.

Ingredient Composition

IngredientPer ServingRaw Material FormAlternative Form (same amount of active)
Lactobacillus Acidophilus10 mg (example amount, powder weight)Freeze-dried Lactobacillus acidophilus culture on a carrier; specification 10 mg of powder per serving, with strain designation, CFU per gram, carrier and water activity set with the supplierThe same culture declared as live cells, for example 2 billion CFU per serving, with the CFU basis stated

The mg amounts on the reference labels are the weight of probiotic powder or lysate and are not live-cell counts, and the CFU amounts are live-cell counts; the two bases are kept apart and are not compared or merged. A brand that wants to state live cells specifies CFU per serving and the basis (at manufacture or at the end of shelf life). The labels name the ingredient as Lactobacillus acidophilus; the strain designation is set in the specification.

Intended Benefits & Formula Rationale

This formula is designed for products positioned around digestive and general wellness probiotics. A single Lactobacillus acidophilus strain is included as the only active so that the strain, the CFU basis and the format can be set around one ingredient.

Intended Benefit DirectionKey IngredientsFormulation Rationale
Probiotic intakeLactobacillus AcidophilusIncluded as a common probiotic species in a single-strain format. Evidence: Product positioning
Urinary tract infections after childbirthLactobacillus AcidophilusIn a double-blind, randomized, placebo-controlled trial of Lactobacillus acidophilus La5 in the puerperium, the incidence of urinary tract infections was significantly lower in the probiotic group at the 2-month follow-up (adjusted odds ratio 0.41, 95% CI 0.17 to 0.93), and the authors recommend further studies [1]. Evidence: Referenced

The example amount is where development starts, and the brand then sets the final amount and the format for its market.

Available Formats

Both a capsule and a tablet are developed from the reference formula, and the brand can adjust counts per serving.

FormatUnitPer ServingPack SizeOther Ingredients (typical)Suitability
Tablet1 tablet10 mg Lactobacillus acidophilus powder60 or 120 tabletsDiluent, binder, glidant, protective coatingCarries the example amount; low-moisture tablet
Capsule1 capsule10 mg Lactobacillus acidophilus powder, or a stated CFU count60 or 90 capsulesCapsule shell, carrier, flow agent, desiccantCommon capsule format among the reference labels

Raw Material Input & Fill (Design Estimate)

Design estimates. The culture is assumed at 100% of the declared weight and a carrier bulk density of 0.6 g/mL, so a milligram-level powder is blended into a carrier to reach a handleable fill.

FormatRaw Material Input per UnitDesign FillBalance for ExcipientsVerification Before Scale-Up
Tablet10 mg culture powderAbout 300 mg tabletAbout 290 mg diluent, binder and glidantCompression effect on viability, hardness, disintegration, CFU count after storage
Capsule10 mg culture powderAbout 250 mg in a size 2 capsuleAbout 240 mg carrier and flow agentBlend uniformity, fill weight variation, CFU count after storage, water activity

Powder sticks, liquids and gummies are separate projects, because live cultures need their own viability study in those formats.

Formulation Considerations

Strain and evidence. Probiotic evidence belongs to a strain and not to a species. The cited trial used Lactobacillus acidophilus La5 [1], so the strain designation is fixed first, and any claim is tied to the strain that is actually used.
CFU basis and overage. The label basis (CFU at manufacture or at end of shelf life) decides the overage in the blend. Viability is counted at release and through the stability plan at the real storage condition.
Moisture and temperature. Freeze-dried cultures lose viability with moisture and heat. Low water activity, a desiccant, blister or alu-barrier packaging and cool storage are specified, and compression can reduce viability in tablets.
Evidence and claims. The cited result comes from a single trial in postpartum women [1], so claims on a finished product are reviewed for each sales market before a product is developed.

Customization Options

Brands adjust the strain, the amount and its basis, and the format.

ParameterRangeNote
Powder per serving (mg basis)0.5 to 500 mg across the reference labelsPowder weight, not live cells
Live cells per serving (CFU basis)20 million to 4 billion CFU across the reference labelsBasis stated: at manufacture or end of shelf life
StrainSet with the supplierEvidence follows the strain
FormatTablet or capsulePowder, liquid and gummy are separate projects
Add-onsOther Lactobacillus or Bifidobacterium strains, prebiotic fiberEach add-on changes the viability plan and the label

The pilot run confirms the CFU count at release and after storage, the water activity of the blend and the tablet hardness or capsule fill weight.

Technical Status & Data Sources

ItemStatusDetails
Reference formulaNIH DSLDThe ingredient and amount come from a labeled product in the NIH DSLD (10 mg on the label of CF002962; example DSLD labels 19172, 65331). The label names the ingredient as Lactobacillus acidophilus; the strain and the CFU basis are set in the specification.
SpecificationSet in formulationLactobacillus acidophilus culture with strain designation, CFU per gram and basis, water activity, carrier, and heavy metal and microbial limits and methods fixed with the supplier. Unit size, fill weight and stability are set in formulation.
Data sourceNIH DSLDDSLD v9.4.0, downloaded 2026-01-10: 50,824 reference formulas merged from on-market labels with complete ingredient amounts. Counts show how labels are distributed.
Market reviewPer marketDose limits, labeling and claims are reviewed for each sales market before a finished product is developed.
ReferencesSources[1] Borhani F, Abbasalizadeh F, Mohammad-Alizadeh-Charandabi S, Homayouni-Rad A, Mirghafourvand M. The Effect of Lactobacillus Acidophilus (La5) on the Incidence of Urinary Tract Infection in the Puerperium: A Randomized Placebo-Controlled Trial. Probiotics Antimicrob Proteins. 2026;18(2):2746-2758. DOI · PubMed

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